C 3: Analysis of regulatory functions of proteasome-associated PRL1 WD-protein and SnRK1 kinases
Dr. Csaba Koncz
Max-Planck-Institut für Pflanzenzüchtungsforschung, Köln
email: koncz@mpipz.mpg.de
phone: +49-(0)221 5062 230
website
Running time: 2003 – 2011
Abstract
The Arabidopsis Pleiotropic Regulatory Locus 1 (PRL1) encodes a nuclear WD-protein which is an inhibitor of AMP-activated SnRK1 kinases in vitro and a binding partner of CDC5 R2R3-Myb factor in a putative spliceosome assembly complex in vivo. PRL1 and CDC5 show proteasomal binding and degradation analogously to SnRK1 kinases that bind the SKP1 subunit of SCF E3 ubiquitin ligases and the ?7/PAD1 subunit of proteasomes. The project aims to explore the role of PRL1 in proteasomal targeting of CDC5 splicing complex and the control of proteolysis of bZIP and IAA/AUX transcription factors by SnRK1 kinases.
(A final report will follow.)
Publications resulting from the project:
Balcerowicz, M., Fittinghoff, K., Wirthmueller, L., Maier, A., Fackendahl, P., Fiene, G., Koncz, C., and Hoecker, U. (2011). Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2. Plant J. 65, 712-23.
Bitrián, M., Roodbarkelari, F., Horvath, M., and Koncz, C. (2011). BAC-recombineering for studying plant gene regulation: developmental control and cellular localization of SnRK1 kinase subunits. Plant J. 65, 829-42.
Henriques, R., Magyar, Z., Monardes, A., Khan, S., Zalejski, C., Orellana, J., Szabados, L., de la Torre, C., Koncz, C., and Bögre, L. (2010). Arabidopsis S6 kinase mutants display chromosome instability and altered RBR1–E2F pathway activity. EMBO J. 29, 2979-2993.
Archacki, R., Sarnowski, T.J., Halibart-Puzio, J., Brzeska, K., Buszewicz, D., Prymakowska-Bosak, M., Koncz, C., and Jerzmanowski, A. (2009). Genetic analysis of functional redundancy of BRM ATPase and ATSWI3C subunits of Arabidopsis SWI/SNF chromatin remodelling complexes. Planta 229, 1281-1292. [last authors with equal contribution]
Kleinow, T., Himbert, S., Krenz, B., Jeske, H., and Koncz, C. (2009). NAC domain transcription factor ATAF1 interacts with SNF1-related kinases and silencing of its subfamily causes severe developmental defects in Arabidopsis. Plant Sci. 177, 360-370.
Kleinridders A, Pogoda, H.M., Irlenbusch, S., Smyth, N., Koncz, C., Hammerschmidt, M., and Brüning, J.C. (2009). PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis. Mol. Cell. Biol. 29, 3173-3185.
Papdi, C., Ábrahám, E., Prathiba Joseph, M., Popescu, C., Koncz, C. and Szabados, L. (2008). Functional identification of Arabidopsis stress regulatory genes with the Controlled cDNA Overexpression System, COS. Plant Physiol. 147, 528-542.
Farkas, I., Dombrádi, V., Miskei, M., Szabados, L., and Koncz, C. (2007). Arabidopsis PPP family of serine/-threonine phosphatases. Trends Plant Sci. 12, 169-176.